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2026 Vol.2, Issue 3 Preview Page

Research Article

Influences of semen extenders and seminal plasma during liquid storage in goat sperm
Nahyeon Kang1
,
Eunju Seok1
,
Jaeuk Cho1
,
Minwoo Sin1
,
Chanjin Woo1
,
Sang-Hee Lee12*
1College of Animal Life Sciences, Kangwon National University, Chuncheon 24341, Republic of Korea
2School of Information and Communication Technology, University of Tasmania, Hobart, TAS7005, Australia
*Corresponding Author
30 September 2026. pp. 142-149
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Abstract

Semen liquid storage is an essential technique for sperm transportation and the preservation of genetic resources for artificial insemination in the goat industry. Maintaining sperm quality during liquid storage is a decisive factor for successful artificial insemination. However, sperm function progressively declines during storage, and the effects of extender, seminal plasma, and temperature on this process remain poorly understood in goat sperm. This study investigated their influence during liquid storage. Semen from four bucks (10.2 ± 2.5 months) was diluted with Modena B (M) or Triladyl (Tr), with (S) or without (NS) seminal plasma, and stored at 4 and 17°C for 120 hours. The viability and acrosome reaction were analyzed by flow cytometry. Viability was significantly higher in Modena B groups (NS M and S M) than in Triladyl groups (NS Tr and S Tr) at 17°C after 72 hours (p < 0.05). The viability of the S M group was significantly higher than that of the S Tr group at 17°C after 120 hours (p < 0.05). The acrosome reaction was significantly higher at 17°C than at 4°C in the NS Tr group at 72 hours (p < 0.05). The acrosome reaction was significantly higher in Triladyl groups (NS Tr and S Tr) than in Modena B groups (NS M and S M) at 17°C after 72 and 120 hours (p < 0.05). In conclusion, the results provide fundamental knowledge regarding long-term liquid preservation techniques for artificial insemination using liquid-preserved goat sperm.

Keywords
Extender
Goat sperm
Liquid preservation
Seminal plasma
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Information
  • Publisher :Journal of Humanimal Sciences
  • Publisher(Ko) :한경국립대학교 휴머니멀응용과학연구소
  • Journal Title :Journal of Humanimal Sciences
  • Journal Title(Ko) :휴머니멀과학학술지
  • Volume : 2
  • No :3
  • Pages :142-149
  • Received Date : 2026-08-13
  • Revised Date : 2026-08-26
  • Accepted Date : 2026-09-01
  • DOI :https://doi.org/10.23341/jhas.2026.2.3.142
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