About This Journal
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Research Article
- Isolation and characterization of lactic acid bacteria from Artichoke (Cynara scolymus L.)
- Narangerel Mijid, Batchimeg Namshir, Urantulkhuur Battumur, Purevsuren Pagam, Oyuntsatsral Batsaikhan, Natsag Lkhagvasuren
- Artichoke (Cynara scolymus L.) contains abundant bioactive compounds, including inulin, phenolic acids, flavonoids, and other phytochemicals that may support the growth …
- Artichoke (Cynara scolymus L.) contains abundant bioactive compounds, including inulin, phenolic acids, flavonoids, and other phytochemicals that may support the growth of beneficial microorganisms. The present study aimed to isolate and characterize lactic acid bacteria (LAB) from fermented artichoke and evaluate their antibacterial activity and safety-related properties. Fermented artichoke leaves were cultured on de Man, Rogosa and Sharpe (MRS) agar, and the selected isolate was identified using API 50 CHL carbohydrate fermentation profiling and 16S rRNA gene sequencing. The isolate was identified as Lactobacillus plantarum with 99.9% sequence similarity. Antibacterial activity of the cell-free supernatant (CFS) was evaluated against Salmonella enteritidis, Staphylococcus epidermidis, Enterococcus faecalis, and Enterococcus casseliflavus using paper disc diffusion and 96-well microplate assays. The strongest inhibitory effects were observed against S. enteritidis, S. epidermidis, and E. faecalis, whereas E. casseliflavus demonstrated relatively lower susceptibility. Hemolytic activity assessment revealed a γ-hemolytic phenotype, indicating the absence of hemolytic activity and supporting the safety of the isolate for food-related applications. These findings demonstrate that artichoke-associated L. plantarum possesses promising antimicrobial properties and may serve as a potential probiotic culture or natural bio-preservative for functional food development. Further studies are required to determine the minimum inhibitory concentration (MIC), characterize the active antimicrobial metabolites, and evaluate probiotic functionality under simulated gastrointestinal conditions. - COLLAPSE
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Research Article
- PCR-RFLP analysis of the callipyge (CLPG) gene in indigenous mongolian sheep breeds
- Myagmarsuren Batdorj, Enkhmaa Shiikhar, Ariunbold Toos Turto, Ulambayar Temuujin, Goomaral Altansukh, Enkhchimeg Vanjildorj, Badamsuren Batsukh
- The callipyge (CLPG) gene is a candidate gene associated with muscle hypertrophy and carcass traits in sheep, influencing growth performance and meat …
- The callipyge (CLPG) gene is a candidate gene associated with muscle hypertrophy and carcass traits in sheep, influencing growth performance and meat quality. The main objective of this study was to investigate CLPG gene polymorphism in seven indigenous Mongolian sheep breeds. A total of 338 blood samples were collected and analyzed using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method with the restriction enzyme BsmFI. Amplification of the target region produced a 426 bp PCR fragment in all samples. Genotyping results revealed only the homozygous AA genotype and the A allele in all individuals examined. These findings indicate that the CLPG/BsmFI polymorphism is monomorphic in the studied Mongolian sheep populations and that the callipyge mutation is absent. Therefore, the CLPG gene does not contribute to genetic variation in muscle hypertrophy and carcass-related traits in these breeds. To our knowledge, this is the first study to investigate CLPG gene polymorphism in indigenous Mongolian sheep breeds. - COLLAPSE
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Research Article
- Comparative effects of sorbitol with red ginger and tussilago-thyme extracts on the growth performance of broiler chickens
- Ma. Teresa D. Santos, Listya Purnamasari, Jeric H. Rosales, Joseph F. dela Cruz
- As technology has led to the development of herbal products that are now commercially available, the use of these products in poultry …
- As technology has led to the development of herbal products that are now commercially available, the use of these products in poultry farming has gained attention due to their potential benefits and convenience. The study evaluated the effects of herbal supplementation of sorbitol with red ginger and tussilago-thyme extracts on the growth performance of broiler chickens. A total of 270 broiler chicks were randomly assigned to three treatment groups, with 10 replicate pens per group and 9 chicks per experimental unit. The treatments were as follows: Control – no supplementation; T1 - sorbitol with red ginger (2g/L); and T2 - tussilago and thyme extract (0.5g/L). The supplements were administered via the drinking water from day 7 to day 35. The production parameters for growth performance that were evaluated are body weight, feed intake, body weight gain, feed conversion ratio (FCR), and livability. Additionally, economic analysis was also assessed using the income over feed and chick cost (IOFCC) computation. Statistical analysis (p<0.05) revealed that herbal supplemented chicks have a significantly higher body weight and body weight gain while having a significantly lower feed intake, compared to the control group. Consequently, the FCR values were more favorable in the supplemented groups. Livability rates throughout the experiment were 96% for T2, 93% for T1, and 66% for the control group, indicating improved survivability in the groups receiving herbal supplementation. Furthermore, IOFCC analysis also highlighted the economic advantages of the two herbal supplements. In conclusion, the tussilago-thyme extract group (T2) achieved the most favorable outcomes across both performance and economic parameters among the three treatments. Implications: These finding highlight the potential of tussilago-thyme extract as a natural growth enhancer in broiler production. Its use could reduce reliance on syntetic growth promoters, promote more sustainable poultry farming practices, and improve both performance and economic returns. - COLLAPSE
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Technical Note
- Seasonal differences in the fatty acid composition of mutton from erdenedalai soum, dundgovi province, mongolia
- Na Qin, Dashdorj Dashmaa, Ravdansuren Chantsaldulam, Bat-Ochir Munkhdelger, Narantuya Solongo, Ariunbold Turtoktokh
- This study comparatively investigated the seasonal differences between winter and spring on fatty acid composition of mutton from Erdenedalai soum in Dundgovi …
- This study comparatively investigated the seasonal differences between winter and spring on fatty acid composition of mutton from Erdenedalai soum in Dundgovi Province, Mongolia. The results showed that oleic acid (C18:1 n-9) was the predominant fatty acid in all samples, accounting for 34.66%–43.57% of total fatty acids. Compared with winter samples, higher contents of palmitic acid (C16:0) and oleic acid were observed in spring samples, whereas butyric acid (C4:0) was markedly higher in winter samples. The lipid health indices indicated that the PUFA/SFA ratio ranged from 1.03 to 1.39, the n-6/n-3 ratio ranged from 1.33 to 1.89, and both the atherogenic index (AI) and thrombogenic index (TI) were at low to moderate levels. Overall, Mongolian mutton exhibited noticeable seasonal variation in fatty acid composition, and its lipid structure demonstrated a relatively balanced nutritional profile. - COLLAPSE
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Technical Note
- Study results on wool yield and selected quality indicators of Mongolian sheep
- Sainchuluu Amarsanaa, Norovsuren Lasran, Narantuya Solongo, Ariunbold Turtogtokh
- This study determined greasy wool yield and selected wool-quality indicators of Mongolian sheep raised in Erdenedalai soum, Dundgovi Province, Mongolia, and compared …
- This study determined greasy wool yield and selected wool-quality indicators of Mongolian sheep raised in Erdenedalai soum, Dundgovi Province, Mongolia, and compared the results with previously reported breed and regional data. The study was conducted in June 2025 using nucleus flocks from the Tsavchir, Tsagaan-Ovoo, Rashaan, and Sangiin Dalai baghs. Greasy wool yield was measured by control shearing in 177 animals, including 53 ewe lamb, 64 ewes, 30 ram lamb, and 30 rams. Mean greasy wool yields were 1.39 ± 0.015 kg, 1.76 ± 0.016 kg, 1.47 ± 0.034 kg, and 1.88 ± 0.052 kg in ewe lamb, ewes, ram lamb, and rams, respectively. Wool composition was analyzed in 22 sheep. Cashmere hair content ranged from 38.37 ± 4.80% to 47.13 ± 4.41%, intermediate hair from 3.90 ± 0.67% to 8.61 ± 3.23%, top hair from 16.58 ± 4.08% to 27.18 ± 5.92%, and coarse hair from 22.74 ± 1.83% to 34.57 ± 3.37%. Cashmere hair diameter was relatively uniform among groups, ranging from 22.30 ± 0.26 to 22.48 ± 0.67 µm, whereas coarse hair diameter reached 53.27 ± 0.86 µm in rams. Cashmere hair length ranged from 4.78 ± 0.08 to 5.99 ± 0.35 cm, and intermediate hair length ranged from 5.62 ± 0.32 to 6.79 ± 0.36 cm. Scurf and extraneous impurity levels were low. The results confirm that the studied Mongolian sheep population is a coarse-wool type and provide baseline information for wool grading, flock selection, and wool-processing practice. - COLLAPSE
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Technical Note
- A wig-based visualization training method for scissor manipulation in dog grooming
- Eun Kyung Kang, Ae Kyung Park
- This technical note proposes a wig-based visualization training method for foundational scissor manipulation in dog grooming. Dog grooming is closely related to …
- This technical note proposes a wig-based visualization training method for foundational scissor manipulation in dog grooming. Dog grooming is closely related to hygiene, coat management, and daily observation of skin condition, but grooming can also expose dogs to unfamiliar handling, restraint, environmental stimuli, tool noise, and blade-related stress. For this reason, novice learners should acquire stable scissoring mechanics before performing fine cutting work on live dogs. The proposed method uses a grooming wig as a controlled pre-live-animal practice surface and focuses on blunt scissors as the basic reference tool for scissoring education. Four visualizable elements are emphasized: fixed-blade stabilization, thumb-driven movement of the moving blade, opening-angle control, and cutting-trajectory alignment on the wig surface. A stepwise training sequence is presented, including grip stabilization without cutting, opening-angle comparison, straight-line cutting, curved-surface adaptation, video feedback, and repeated correction. To improve reproducibility, the method also requires instructors to record the wig type, fixation method, scissor type, marking method, session duration, repetition scheme, and assessment criteria used in each class. This technical note does not report experimental efficacy data; rather, it provides a practical framework for converting tacit grooming skills into observable training criteria, reducing unnecessary early live-dog exposure, and supporting future studies on learner performance and transfer to supervised live-dog practice. - COLLAPSE
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Review Article
- Antiproliferative effects and preclinical limitations of quercetin in hepatocellular carcinoma
- Ji-Yeon Mo, Ho-Jun Lee
- Hepatocellular carcinoma (HCC) remains a major clinical burden because tumor heterogeneity, underlying liver dysfunction, recurrence, and variable therapeutic responses continue to limit …
- Hepatocellular carcinoma (HCC) remains a major clinical burden because tumor heterogeneity, underlying liver dysfunction, recurrence, and variable therapeutic responses continue to limit durable disease control. Quercetin, a dietary flavonol, has been repeatedly investigated in HCC-related experimental systems, but its interpretation requires caution because most evidence remains preclinical and model-dependent. This review reinterprets quercetin-associated HCC evidence around a central mechanistic axis: redox regulation, p53/BAX-caspase-dependent apoptosis, survival signaling suppression, cell-cycle control, and lipid metabolic vulnerability. In HepG2-centered studies, quercetin reduced proliferation and induced apoptosis under time- and dose-dependent conditions, with reported changes in intracellular reactive oxygen species, caspase-3/9 activation, PI3K/Akt and ERK signaling, cyclin-related cell-cycle regulation, and fatty acid synthase activity. Comparative data, however, indicate that HCC cell lines do not respond uniformly; the limited response of Huh7 cells and the hepatoblastoma-derived origin of HepG2 restrict broad generalization to human HCC. Combination studies with cisplatin or 5-fluorouracil suggest experimental chemosensitization, not established clinical combination therapy. In vivo data from HepG2 xenografts support tumor-growth delay, but do not resolve pharmacokinetic, immune microenvironment, toxicity, and bioavailability limitations. Overall, quercetin is best interpreted as a multi-target preclinical candidate that may expose redox and metabolic vulnerabilities in selected HCC models, rather than as a validated HCC treatment. - COLLAPSE
Journal of Humanimal Sciences
