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2026 Vol.2, Issue 2 Preview Page

Research Article

PCR-RFLP analysis of the callipyge (CLPG) gene in indigenous mongolian sheep breeds
Myagmarsuren Batdorj1
,
Enkhmaa Shiikhar13
,
Ariunbold Toos Turto2
,
Ulambayar Temuujin1
,
Goomaral Altansukh1
,
Enkhchimeg Vanjildorj1
,
Badamsuren Batsukh1*
1Department of Biotechnology and Breeding, Mongolian University of Life Sciences, Ulaanbaatar 17024, Mongolia
2Department of Animal Husbandry and Technology, Mongolian University of Life Sciences, Ulaanbaatar 17024, Mongolia
3Department of Pharmacy and Biomedicine, Mongolian National University of Medical Sciences, Dornogobi province 44105, Mongolia
*Corresponding Author
30 June 2026. pp. 81-86
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Abstract

The callipyge (CLPG) gene is a candidate gene associated with muscle hypertrophy and carcass traits in sheep, influencing growth performance and meat quality. The main objective of this study was to investigate CLPG gene polymorphism in seven indigenous Mongolian sheep breeds. A total of 338 blood samples were collected and analyzed using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method with the restriction enzyme BsmFI. Amplification of the target region produced a 426 bp PCR fragment in all samples. Genotyping results revealed only the homozygous AA genotype and the A allele in all individuals examined. These findings indicate that the CLPG/BsmFI polymorphism is monomorphic in the studied Mongolian sheep populations and that the callipyge mutation is absent. Therefore, the CLPG gene does not contribute to genetic variation in muscle hypertrophy and carcass-related traits in these breeds. To our knowledge, this is the first study to investigate CLPG gene polymorphism in indigenous Mongolian sheep breeds.

Keywords
Callipyge gene (CLPG)
Genetic polymorphism
Indigenous Mongolian sheep breeds
PCR-RFLP
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Information
  • Publisher :Journal of Humanimal Sciences
  • Publisher(Ko) :한경국립대학교 휴머니멀응용과학연구소
  • Journal Title :Journal of Humanimal Sciences
  • Journal Title(Ko) :휴머니멀과학학술지
  • Volume : 2
  • No :2
  • Pages :81-86
  • Received Date : 2026-06-11
  • Revised Date : 2026-06-15
  • Accepted Date : 2026-06-15
  • DOI :https://doi.org/10.23341/jhas.2026.2.2.81
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