All Issue

2026 Vol.2, Issue 2

Research Article

Isolation and characterization of lactic acid bacteria from Artichoke (Cynara scolymus L.)
Narangerel Mijid1
,
Batchimeg Namshir1
,
Urantulkhuur Battumur1
,
Purevsuren Pagam1
,
Oyuntsatsral Batsaikhan2
,
Natsag Lkhagvasuren13*
1School of Animal Science and Biotechnology, Mongolian University of Life Sciences, Ulaanbaatar 17029, Mongolia
2Research Institute of Animal Husbandry, Ulaanbaatar 17024, Mongolia
3Livestock Technology Advancement Center, Mongolian University of Life Sciences, Ulaanbaatar 17029, Mongolia
*Corresponding Author
30 June 2026. pp. 69-80
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Abstract

Artichoke (Cynara scolymus L.) contains abundant bioactive compounds, including inulin, phenolic acids, flavonoids, and other phytochemicals that may support the growth of beneficial microorganisms. The present study aimed to isolate and characterize lactic acid bacteria (LAB) from fermented artichoke and evaluate their antibacterial activity and safety-related properties. Fermented artichoke leaves were cultured on de Man, Rogosa and Sharpe (MRS) agar, and the selected isolate was identified using API 50 CHL carbohydrate fermentation profiling and 16S rRNA gene sequencing. The isolate was identified as Lactobacillus plantarum with 99.9% sequence similarity. Antibacterial activity of the cell-free supernatant (CFS) was evaluated against Salmonella enteritidis, Staphylococcus epidermidis, Enterococcus faecalis, and Enterococcus casseliflavus using paper disc diffusion and 96-well microplate assays. The strongest inhibitory effects were observed against S. enteritidis, S. epidermidis, and E. faecalis, whereas E. casseliflavus demonstrated relatively lower susceptibility. Hemolytic activity assessment revealed a γ-hemolytic phenotype, indicating the absence of hemolytic activity and supporting the safety of the isolate for food-related applications. These findings demonstrate that artichoke-associated L. plantarum possesses promising antimicrobial properties and may serve as a potential probiotic culture or natural bio-preservative for functional food development. Further studies are required to determine the minimum inhibitory concentration (MIC), characterize the active antimicrobial metabolites, and evaluate probiotic functionality under simulated gastrointestinal conditions.

Keywords
Antibacterial activity
Cell-free supernatant
Cynara scolymus L.
Lactic acid bacteria
Lactobacillus plantarum
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Information
  • Publisher :Journal of Humanimal Sciences
  • Publisher(Ko) :한경국립대학교 휴머니멀응용과학연구소
  • Journal Title :Journal of Humanimal Sciences
  • Journal Title(Ko) :휴머니멀과학학술지
  • Volume : 2
  • No :2
  • Pages :69-80
  • Received Date : 2026-06-08
  • Accepted Date : 2026-06-10
  • DOI :https://doi.org/10.23341/jhas.2026.2.2.69
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