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2025 Vol.1, Issue 1

Research Article

Morphological changes of colorectal cancer cells and the effects of apple-derived dietary fiber in a 3D culture system using a collagen matrix
Min-Jee Oh12†
,
Sang-Hwan Kim12†*
1School of Animal Life Science, Hankyong National University, Anseong 17579, Republic of Korea
2Institute of Applied Humanimal Science, Hankyong National University, Anseong 17579, Republic of Korea
*Corresponding Author
†These authors contributed equally to this work.
31 March 2025. pp. 1-9
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Abstract

Colorectal cancer (CRC) is a leading cause of cancer-related mortality, with metastasis being the primary cause of death. Current in vitro models fail to fully replicate the tumor microenvironment (TME). This study developed a collagen matrix-based 3D culture system using HCT116 CRC cells to investigate tumor-stroma interactions. Four culture types were prepared, with Type 4 incorporating apple-derived dietary fiber. 17β- estradiol (10⁻⁸ M) and EGF (0.01 ng/mL) were added to assess their effects. Phalloidin staining confirmed cytoskeletal remodeling, with Type 4 showing the most pronounced structural organization. Indicated that hormones and dietary fiber synergistically enhanced tumor formation. These findings highlight the importance of 3D culture models in CRC research and suggest dietary components may influence tumor behavior, providing a novel tool for studying CRC progression.

Keywords
3D cell culture
Apple-derived dietary fiber
Collagen matrix
Colorectal cancer
Morphological changes
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Information
  • Publisher :Journal of Humanimal Sciences
  • Publisher(Ko) :한경국립대학교 휴머니멀응용과학연구소
  • Journal Title :Journal of Humanimal Sciences
  • Journal Title(Ko) :휴머니멀과학학술지
  • Volume : 1
  • No :1
  • Pages :1-9
  • Received Date : 2025-02-10
  • Revised Date : 2025-02-13
  • Accepted Date : 2025-02-18
  • DOI :https://doi.org/10.23341/jhas.2025.1.1.1
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