Comparison of genetic diversity for differential gene expression analysis associated with bovine embryo quality

Xu Wenchao; Enkhbolor Barsuren

doi:10.23341/jhas.2026.2.1.1

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2026 Vol.2, Issue 1 Next Page

Research Article

Comparison of genetic diversity for differential gene expression analysis associated with bovine embryo quality

31 March 2026. pp. 1-9

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Abstract

Embryo quality is a key driver of multiple ovulation and embryo transfer (MOET) performance and is shaped by both the uterine milieu and endocrine signaling. This study tested whether donor cows classified by embryo quality at day 7 after artificial insemination (AI) could be discriminated using cDNA fingerprinting derived from endometrial epithelial cells (EECs). Embryos and EECs were recovered by nonsurgical uterine lavage, and embryos were graded using International Embryo Technology Society (IETS) criteria. Donors were grouped according to embryo quality outcome (good, fair, and bad), and random amplified polymorphic DNA PCR (RAPD-PCR) was conducted with 11 UFPF primers on EEC-derived cDNA. In parallel, follicle-stimulating hormone (FSH), luteinizing hormone (LH), and pregnancy-associated plasma protein A (PAPP-A) in uterine fluid were quantified by ELISA. RAPD banding profiles, combined with UPGMA-based clustering, yielded distinct patterns that separated EEC groups. Endocrine profiling further indicated reduced FSH and PAPP-A in uterine fluid from the outcome group relative to higher-quality groups. Collectively, EEC cDNA fingerprinting together with uterine endocrine measures may serve as complementary indicators associated with bovine embryo quality, although marker identification beyond pattern-based discrimination remains necessary.

Keywords

Bovine

Embryo quality

Endometrial epithelial cells

FSH

PAPP-A

RAPD-PCR

Uterine fluid

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Information

Publisher :Journal of Humanimal Sciences
Publisher(Ko) :한경국립대학교 휴머니멀응용과학연구소
Journal Title :Journal of Humanimal Sciences
Journal Title(Ko) :휴머니멀과학학술지
Volume : 2
No :1
Pages :1-9
Received Date : 2026-01-16
Revised Date : 2026-02-12
Accepted Date : 2026-02-13
DOI :https://doi.org/10.23341/jhas.2026.2.1.1

Journal of Humanimal Sciences ISSN:3091-5600(Print) 3091-5716(Online) 휴머니멀과학학술지

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